Leading In Vitro Cell Cultlure Techniques

 Biokeystone      

   the coverslip coating expert

Get advanced cell culture techniques

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      Biokeystone      

 Providing quality products since 1994

 

Frequent Asked Questions

 

How to enhance poly-L-lysine coating for cell culture?

 

Poly-L-lysine coating is one of the most critical factors for the success of cell culture. The major considerations of the best poly-L-lysine coating are:

  • The molecular weight of the poly-L-lysine used for coating: Longer poly-lysine molecule provides much more cell binding sites on a given culture surface.

  • The concentration of the poly-L-lysine in coating: Higher concentration can saturate the culture surface.

  • Rinse off floating poly-L-lysine after coating to ensure uniform extracellular matrix for consistent cell culture.

 

  Coated, sterile coverslips

Coated with poly-L-lysine (PLL), poly-D-lysine (PDL), rat tail collagen, tested for primary cell and neuron culture, starting 12 mm coverslips $54.75/box

 

Biokeystone Product lines

 

neuVitro Products

 

  German glass coverslips 

Tested for primary cell and primary neuron cultures, starting $12/box

 

 

  MCI: Microfluid neuron culture

Generating robust cell cultures easily. Especially powerful for primary neuron cultures, poly-L-lysine coated, optically clear, sterile, ready to use

 

6mgel Products

 

Run gel in dd-water

See you PCR and genotyping results in 6 min with straight banding technology

 

 

  Rapid Genotyping system

One-step DNA extraction from tissue to genotyping PCR, no spin no purification