principle

Principle of high speed gel electrophoresis (US patent 5,549,806)

Conventional setting of

gel electrophoresis

In conventional gel devices, gel body is immersed in running buffer. This setting format determines submarine agarose gel electrophoresis a slow process because:

Running buffer, a highly conductive liquid, conducts heavy electric current which leads to massive heat generation.

Sample migration pattern will be distorted and the gel body could be melted at high temperature, resulting in poor gel quality

High speed setting of gel electrophoresis

he breakthrough of high speed agarose gel electrophoresis system: gel body is immersed in deionized water. This simple one step addition gains surprising advantages:

Deionized water, an electric insulator, suppresses electric current which prevents massive heat generation.

Water, the most potent coolant, absorbs heat from gel body efficiently and evenly so that uniform low temperature among gel body is maintained during agarose gel electrophoresis.

Much higher voltage, such as 280V, can be applied to drive rapid sample migration and form sharp bands across sample lanes. Sample diffusion is substantially reduced during agarose gel electrophoresis.

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